Thermo Fisher Announces New Method
Thermo Fisher Scientific recently announced a new method that can automatically extract glycoproteins from samples, and glycopeptides from tryptic digests, thus enabling faster isolation, better sample recovery, high peak efficiency, and high-throughput capability. Application Note (AN) 1014: Concanavalin A Column for Analysis of Glycoproteins and Their Tryptic Glycopeptides demonstrates that this approach enhances efficiency by leveraging innovative products. For example, this new method uses a high-performance liquid chromatography (HPLC)-compatible Concanavalin A (Con A) column that is built on a monolithic supportand offers advantages over other Con A columns.
AN 1014 also shows that a fully biocompatible analytical LC system can automate the entire off-line process, from sample enrichment and fraction collection to the reanalysis of collected sample by peptide mapping with both ultraviolet and mass spectrometry detections. Monitoring oxonium ions with a single quadrupole mass spectrometer is a selective, sensitive, and reliable method that confirms the identity of the glycopeptides captured by the Con A column. This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
Thermo Fisher Scientific Inc. is a world leader in serving science. Its mission is to enable customers to make the world healthier, cleaner, and safer. With revenues of $12 billion, it has approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions, and government agencies, as well as in environmental and process control industries. For more information, visit www.thermofisher.com.
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