Thermo Fisher Scientific Uses New Method to Determine Sialic Acids in Glycoproteins
Thermo Fisher Scientific recently announced a new method that uses high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) to determine sialic acids in glycoproteins. Application Update 181: Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD demonstrates good recoveries, precision, and linear detection for N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). Using the Thermo Scientific Dionex CarboPac PA20 Fast Sialic Acid column, this rapid method separates Neu5Ac and Neu5Gc with a total analysis time of < 5 minutes, providing high-throughput sample analysis while reducing eluent consumption and waste generation.
Glycoprotein sialylation is critical to bioavailability, stability, metabolism, and immunogenicity of therapeutic proteins. As a result, proteins such as Neu5Ac and Neu5Gc are routinely analyzed to determine sialylation amount and identity.
This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
Thermo Fisher Scientific Inc. is a world leader in serving science with a mission to enable its customers to make the world healthier, cleaner, and safer. With revenues of nearly $11 billion, it has approximately 37,000 employees and serves customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries.
Total Page Views: 782
